Founded in 1975 as the all Union Research Institute of Applied Enzimology, currently,  the Institute of Biotechnology is mainly involved in research and training in the fields of biotechnology and molecular biology, including research and development of recombinant biomedical proteins, genetic and molecular studies of restriction modification phenomenon, developing of viruses diagnostics, epigenetic study of small RNA, drug design and synthesis, bioinformatics.

Laboratory of Bioinformatics • Software • PSI-BLAST-ISS

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The PSI-BLAST Intermediate Sequence Search tool: PSI-BLAST-ISS

The Intermediate Sequence Search (ISS) tool is designed to assess the region-specific alignment reliability between two or more protein sequences (target and template(s)). The main idea of the algorithm is to initiate additional PSI-BLAST searches against the non-redundant sequence database for a set of sequences that are related both to the target and to the template(s). The position- specific reliability of the alignment between the target and the template(s) is then assessed by merging alignment data obtained from intermediate sequence searches and analyzing alignment convergence.

Download the recent version of the psi-blast-iss tool,
iss-v1.04.tar.gz [version 1.04]
The older versions:
iss-v1.03.tar.gz [version 1.03]
iss-v1.02.tar.gz [version 1.02]
iss-v1.01.tar.gz [version 1.01]
iss-v1.0.tar.gz [version 1.0]
To install the tool, download the package to your local directory, uncompress and untar it by running the command
tar -zxvf iss-v<ver_num>.tar.gz
Then follow the instructions in the README.install file.
References:
Margelevicius M., Venclovas C. (2005) PSI-BLAST-ISS: an intermediate sequence search tool for estimation of the position-specific alignment reliability. BMC Bioinformatics 6(1):185