Founded in 1975 as the all Union Research Institute of Applied Enzimology, currently,  the Institute of Biotechnology is mainly involved in research and training in the fields of biotechnology and molecular biology, including research and development of recombinant biomedical proteins, genetic and molecular studies of restriction modification phenomenon, developing of viruses diagnostics, epigenetic study of small RNA, drug design and synthesis, bioinformatics.

Publications (2011-2013)

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  1. Horvath, P., Gasiunas, G., Siksnys, V. & Barrangou, R., Applications of the Versatile CRISPR-Cas Systems , 2013, 267-286. (PDF)
  2. Sinkunas, T., Gasiunas, G., Waghmare, S. P., Dickman, M. J., Barrangou, R., Horvath, P. & Siksnys, V., In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO J, 2013. (PDF)
  3. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V., Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A, 2012, vol. 109, E2579-E2586. (PDF)
  4. Manakova, E., Grazulis, S., Zaremba, M., Tamulaitiene, G., Golovenko, D. & Siksnys, V., Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease. Nucleic Acids Res, 2012, vol. 40, 6741-6751. (PDF)
  5. Silanskas, A., Zaremba, M., Sasnauskas, G. & Siksnys, V., Catalytic activity control of restriction endonuclease--triplex forming oligonucleotide conjugates. Bioconjug Chem, 2012, vol. 23, 203-211. (PDF)
  6. Sukackaite, R., Grazulis, S., Tamulaitis, G. & Siksnys, V., The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine. Nucleic Acids Res, 2012, vol. 40, 7552-7562. (PDF)
  7. Zaremba, M., Sasnauskas, G. & Siksnys, V., The link between restriction endonuclease fidelity and oligomeric state: a study with Bse634I. FEBS Lett, 2012, vol. 586, 3324-3329. (PDF)
  8. Kostiuk, G., Sasnauskas, G., Tamulaitiene, G. & Siksnys, V., Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease. Nucleic Acids Res, 2011, vol. 39, 3744-3753. (PDF)
  9. Sapranauskas, R., Gasiunas, G., Fremaux, C., Barrangou, R., Horvath, P. & Siksnys, V., The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res, 2011, vol. 39, 9275-9282. (PDF)
  10. Sasnauskas, G., Kostiuk, G., Tamulaitis, G. & Siksnys, V., Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation. Nucleic Acids Res, 2011, vol. 39, 8844-8856. (PDF)
  11. Silanskas, A., Foss, M., Wende, W., Urbanke, C., Lagunavicius, A., Pingoud, A. & Siksnys, V., Photocaged variants of the MunI and PvuII restriction enzymes. Biochemistry, 2011, vol. 50, 2800-2807. (PDF)
  12. Sinkunas, T., Gasiunas, G., Fremaux, C., Barrangou, R., Horvath, P. & Siksnys, V., Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system. EMBO J, 2011, vol. 30, 1335-1342. (PDF)