Founded in 1975 as an independent research institution, now part of Vilnius University, the Institute of Biotechnology strives to maintain the high standards of excellence in scientific endeavour, research training and technological advance with its main focus ina broadly defined field of molecular biotechnology including nucleic acid and protein technologies, bioinformatics, molecular diagnostics, drug design, next generation epigenomic and gene editing technologies. The Institute provides an interface between advanced education, basic research and technological development for the economic and social benefit of Lithuania.

Publications (2011-2014)


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  1. Gasiunas, G., Sinkunas, T. & Siksnys, V., Molecular mechanisms of CRISPR-mediated microbial immunity Cell Mol. Life Sci., 2014, vol. 71, 449-465. (PDF)
  2. Golovenko, D., Manakova, E., Zakrys, L., Zaremba, M., Sasnauskas, G., Gražulis, S. & Siksnys, V., Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme Nucleic Acids Research, 2014. (PDF)
  3. Gasiunas, G. & Siksnys, V., RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing? Trends Microbiol., 2013, vol. 21, 562-7. (PDF)
  4. Horvath, P., Gasiunas, G., Siksnys, V. & Barrangou, R., Applications of the Versatile CRISPR-Cas Systems , 2013, 267-286. (PDF)
  5. Karvelis, T., Gasiunas, G., Miksys, A., Barrangou, R., Horvath, P. & Siksnys, V., crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus RNA Biol., 2013, vol. 10, 841-51. (PDF)
  6. Karvelis, T., Gasiunas, G. & Siksnys, V., Programmable DNA cleavage in vitro by Cas9 Biochem. Soc. Trans., 2013, vol. 41, 1401-6. (PDF)
  7. Sinkunas, T., Gasiunas, G., Waghmare, S. P., Dickman, M. J., Barrangou, R., Horvath, P. & Siksnys, V., In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO J, 2013. (PDF)
  8. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V., Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A, 2012, vol. 109, E2579-E2586. (PDF)
  9. Manakova, E., Grazulis, S., Zaremba, M., Tamulaitiene, G., Golovenko, D. & Siksnys, V., Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease. Nucleic Acids Res, 2012, vol. 40, 6741-6751. (PDF)
  10. Silanskas, A., Zaremba, M., Sasnauskas, G. & Siksnys, V., Catalytic activity control of restriction endonuclease--triplex forming oligonucleotide conjugates. Bioconjug Chem, 2012, vol. 23, 203-211. (PDF)
  11. Sukackaite, R., Grazulis, S., Tamulaitis, G. & Siksnys, V., The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine. Nucleic Acids Res, 2012, vol. 40, 7552-7562. (PDF)
  12. Zaremba, M., Sasnauskas, G. & Siksnys, V., The link between restriction endonuclease fidelity and oligomeric state: a study with Bse634I. FEBS Lett, 2012, vol. 586, 3324-3329. (PDF)
  13. Kostiuk, G., Sasnauskas, G., Tamulaitiene, G. & Siksnys, V., Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease. Nucleic Acids Res, 2011, vol. 39, 3744-3753. (PDF)
  14. Sapranauskas, R., Gasiunas, G., Fremaux, C., Barrangou, R., Horvath, P. & Siksnys, V., The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res, 2011, vol. 39, 9275-9282. (PDF)
  15. Sasnauskas, G., Kostiuk, G., Tamulaitis, G. & Siksnys, V., Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation. Nucleic Acids Res, 2011, vol. 39, 8844-8856. (PDF)
  16. Silanskas, A., Foss, M., Wende, W., Urbanke, C., Lagunavicius, A., Pingoud, A. & Siksnys, V., Photocaged variants of the MunI and PvuII restriction enzymes. Biochemistry, 2011, vol. 50, 2800-2807. (PDF)
  17. Sinkunas, T., Gasiunas, G., Fremaux, C., Barrangou, R., Horvath, P. & Siksnys, V., Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system. EMBO J, 2011, vol. 30, 1335-1342. (PDF)