Founded in 1975 as the all Union Research Institute of Applied Enzimology, currently,  the Institute of Biotechnology is mainly involved in research and training in the fields of biotechnology and molecular biology, including research and development of recombinant biomedical proteins, genetic and molecular studies of restriction modification phenomenon, developing of viruses diagnostics, epigenetic study of small RNA, drug design and synthesis, bioinformatics.

Publications

Publications (2003-2009)

Zvirbliene A, Kucinskaite-Kodze I, Juozapaitis M, Lasickiene R, Gritenaite D, Russell G, Bingham J, Michalski WP, Sasnauskas K. Novel monoclonal antibodies against Menangle virus nucleocapsid protein. Arch Virol. 2009 Nov 8.  

Zvirbliene A, Sezaite I, Pleckaityte M, Kucinskaite-Kodze I, Juozapaitis M, Sasnauskas K. Mapping of an antigenic site on the nucleocapsid protein of human parainfluenza virus type 3. Viral Immunol. 2009; 22:181-188.

Ebbesen P, Pettersen EO, Gorr TA, Jobst G, Williams K, Kieninger J, Wenger RH, Pastorekova S, Dubois L, Lambin P, Wouters BG, Van Den Beucken T, Supuran CT, Poellinger L, Ratcliffe P, Kanopka A, Görlach A, Gasmann M, Harris AL, Maxwell P, Scozzafava A. Taking advantage of tumor cell adaptations to hypoxia for developing new tumor markers and treatment strategies. J Enzyme Inhib Med Chem. 2009. Suppl 1: 1-39. 

D.Dorn, R.Lawatchek, A.Zvirbliene, E.Aleksaite, G.Petcher, K.Sasnauskas, M.Ozel, M.Raftery, G.Schonrich, R.Ulrich, A.Gedvilaite. Cellular and humoral immunogenicity of hamster polyomavirus-derived virus-like particles harboring a mucin 1 cytotoxic T-cell epitope. Viral Immunol, 2008; 21: 12-27.

M.Juozapaitis, A.Zvirbliene, I.Kucinskaite, I.Sezaite, R.Slibinskas, M.Coiras, F. de Ory Manchon, M.López-Huertas, P.Perez-Brena, J.Staniulis, I.Narkeviciute, K.Sasnauskas. Synthesis of recombinant human parainfluenza virus 1 and 3 nucleocapsid proteins in yeast Saccharomyces cerevisiae. Virus Res. 2008. 133, 178-186.

Makino Y., Uenishi R, Okamoto K., Isoe T., Hosono O., Tanaka O., Tanaka H., Kanopka A.,.Poellinger L., Haneda L., Morimoto C. Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells. J Biol Chem. 2007, 282(19): 14073-14082.

R.Petraityte, L.Jin, R.Hanjan, A.Razanskiene, A.Zvirbliene, K.Sasnauskas. Detection of Hantaan Virus-Specific IgG and IgM in Oral Fluid Using Yeast Expressed Recombinant Nucleocapsid Protein. Clin Vaccine Immunol. 2007; 14, 1603-1608.

M.Juozapaitis, A.Serva, I.Kucinskaite, A.Zvirbliene, R.Slibinskas, J.Staniulis, K.Sasnauskas, B.J.Shiell, T.R.Bowden, W.P.Michalski. Generation of menangle virus nucleocapsid-like particles in yeast Saccharomyces cerevisiae. J.Biotechnol. 2007, 130: 441-447.

I.Kucinskaite, M.Juozapaitis, A.Serva, A.Zvirbliene, N.Johnson, J.Staniulis, A.R.Foks, T.Muller, K.Sasnauskas, R.Ulrich. Antigenic characterization of yeast-expressed lyssavirus nucleoproteins. Virus Genes, 2007, 35: 521-529.

M.Zaveckas, A.Zvirbliene, G.Zvirblis, V.Chmieliauskaite, V.Bumelis, H.Pesliakas. Effect of surface histidine mutations and their number on the partitioning and refolding of recombinant human granulocyte-colony stimulating factor (Cys17Ser) in aqueous two-phase systems containing chelated metal ions. J Chromatogr B Analyt Technol Biomed Life Sci. 2007; 852 (1-2):409-419.

M.Juozapaitis, A.Serva, A.Zvirbliene, R.Slibinskas, J.Staniulis, K.Sasnauskas, B.J.Shiell, L.F.Wang, W.P.Michalski. Generation of henipavirus nucleocapsid proteins in yeast Saccharomyces cerevisiae. Virus Res., 2007, 124: 95-102.

Zvirbliene A., Kucinskaite I., Sezaite I., Samuel D., Sasnauskas K. Mapping of B cell epitopes in measles virus nucleocapsid protein., Arch Virol. 2007, 152: 25-39

I.Kucinskaite, R.Petraityte, I.Sezaite, K.Sasnauskas, A.Zvirbliene. Immunogenicity study of recombinant rubella virus capsid protein and generation of monoclonal antibodies. Biologija, 2006, 2, 72-75.

Samonskyte L., Kucinskaite I., Gedvilaite A., Sasnauskas K., Zvirbliene A. Immunogenic properties of polyomavirus-derived recombinant virus-like particles: activation of murine spleen-cell derived dendritic cells, Biologija, 2006, 2, 80-84.

Bierman J., Lang D.,Gorboulev V., Koepsell H., Sindic A., Schroeter R., Zvirbliene A., Pavenstead H., Schlatter E., Ciaramboli G. Characterization of regulatory mechanisms and states of human organic cation transporter 2. Am J Physiol Cell Physiol. 2006, 290; C1521-C1531.

E.V.Kashuba, K.Gradin, M.Isaguliants, L.Szekely, L.Poellinger, G.Klein, A.Kazlauskas. Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. J Biol Chem., 2006, 281, 1215-1223

J.Flanagan, V.Popendikyte, N.Pozdniakovaite, M.Sobolev, A.Assadzadeh, A.Schumacher, M. Zangeneh, L.Lau, C.Virtanen, S.Chong Wang, and A.Petronis. Intra- and Inter-Individual Epigenetic Variation in Human Germ Cells. Am J Hum Genet. 2006;79:67-84.

A.Zvirbliene, L. Samonskyte, A. Gedvilaite, T. Voronkova, R. Ulrich, K. Sasnauskas. Generation of monoclonal antibodies of desired specificity using chimeric polyomavirus-derived virus-like particles. J Immunol Meth., 2006; 311, 57-70

A.Petronis, I.I. Gotesman, P.Kan, J.L.Kennedy, V.S.Basile, A.D.Peterson, V.Popendikyte. Monozygotic twins exhibit numerous epigenetic differences: clues to twin discordance? 2003 Schizophrenia Bulletin, 29 (1): 169-178

D.Bakonyte, A.Baranauskaite, J.Cicenaite, A.Sosnovskaja, P.Stakenas. Molecular characterization of isoniazid-resistant Mycobacterium tuberculosis clinical isolates in Lithuania. Antimicrob. Agents and Chemother, 2003, 47:2009-2011.

D.Samuel, K.Sasnauskas, L.Jin, A.Gedvilaite, R.Slibinskas, S.Beard, A.Zvirbliene, S.A.Oliveira, J.Staniulis, B.Cohen, D.Brown. Development of a measles specific IgM ELISA for use with serum and oral fluid samples using recombinant measles nucleoprotein produced in Saccharomyces cerevisiae. 2003. J. Clin. Virol., 28:121-129

R.Slibinskas, A.Zvirbliene, A.Gedvilaite, D.Samuel, L.Jin, S.Beard, J.Staniulis, K.Sasnauskas. 2003. Synthesis of mumps virus nucleocapsid protein in yeast Pichia pastoris. J. Biotechnology, 103:43-49.

Patent application:
Žvirblienė A., Gedvilaitė A., Ulrich, R., Sasnauskas K. Process for the production of monoclonal antibodies using chimeric VLPs. Patent Application No. PCT/EP2006/003420, Publication No. WO2006/108658.

Patent:
A.Zvirbliene, A.Gedvilaite, R.Ulrich, K.Sasnauskas. A mean to produce monoclonal antibodies; hybridoma cell lines generated by this mean, and recombinant chimeric virus-like particles with inserted foreign protein fragments as immunogens to generate hybridomas by this mean. Patent No. 5414 (Lithuanian Patent Office, 25.04.2007).

Project

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  • Department of Protein - DNA Interactions
  • Department of Biological DNA Modification
  • Department of Biothermodynamics and Drug Design
  • Department of Eukaryote Gene Engineering
  • Department of Immunology and Cell Biology
    • Antigenicity studies of recombinant viral proteins and development of monoclonal antibodies
    • Molecular epidemiology of Mycobacterium tuberculosis
    • Regulation of gene expression by alternative splicing

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  • Department of Bioinformatics
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